The Transcription Termination Region of the Adenovirus 2 Major Late Transcript Contains Multiple Functional Elements

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15 صفحه اول

A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor.

A simple approach has been developed for the unambiguous identification and purification of sequence-specific DNA-binding proteins solely on the basis of their ability to bind selectively to their target sequences. Four independent methods were used to identify the promoter-specific RNA polymerase II transcription factor MLTF as a 46-kilodalton (kDa) polypeptide. First, a 46-kDa protein was spe...

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Generation and functional analyses for base-substitution mutants of the adenovirus 2 major late promoter.

The function of guanosine residues surrounding the TATA box of the adenovirus 2 major late promoter (MLP) in promoting efficient transcription initiation and in selecting a specific transcription start site in vitro has been examined. Multiple and single base substitutions (G----A) were generated in this region (from -63 to +25 relative to the cap site, +1) of the MLP. The promoter activities o...

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ژورنال

عنوان ژورنال: Journal of General Virology

سال: 1989

ISSN: 0022-1317,1465-2099

DOI: 10.1099/0022-1317-70-6-1337